Flow cytometry: Surface markers and beyond

Genetic Variation of Lime (Citrus sp.) Accessions Using Flow Cytometry Technique, Morphological Characteristics and Molecular Markers

Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-descri...

Phosphoproteins by Flow Cytometry Surface Markers and Intracellular Coordinate Analysis of Murine Immune Cell

Sperm Flow Cytometry: Beyond Human Fertilization and Embryo Development

Male infertily is a contributing factor in up to 50% of all infertility cases, a solo cause in about 30% of them. Therefore, new and improved diagnostic methods that reduce operator variability regarding sperm defects that are not accesible by the conventional microscope scoring should be evaluated. Assisted reproductive technology (ART) has been involved in the description of alternative pathw...

Optimized Surface Markers for the Prospective Isolation of High-Quality hiPSCs using Flow Cytometry Selection

hiPSC derivation and selection remains inefficient; with selection of high quality clones dependent on extensive characterization which is not amenable to high-throughput (HTP) approaches. We recently described the use of a cocktail of small molecules to enhance hiPSC survival and stability in single cell culture and the use of flow cytometry cell sorting in the HTP-derivation of hiPSCs. Here w...

Flow Cytometry

Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...